NAE1 Stable Expression Cell Line (HuH-7)

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  • 细胞图0 NAE1 Stable Expression Cell Line (HuH-7)-细胞图0
  • 细胞图1 NAE1 Stable Expression Cell Line (HuH-7)-细胞图1
  • 质粒图0 NAE1 Stable Expression Cell Line (HuH-7)-质粒图0
  • NAE1 Stable Expression Cell Line (HuH-7) NAE1 Stable Expression Cell Line (HuH-7)-产品图
细胞图0 NAE1 Stable Expression Cell Line (HuH-7)-细胞图0
细胞图1 NAE1 Stable Expression Cell Line (HuH-7)-细胞图1
质粒图0 NAE1 Stable Expression Cell Line (HuH-7)-质粒图0
NAE1 Stable Expression Cell Line (HuH-7) NAE1 Stable Expression Cell Line (HuH-7)-产品图
NAE1 Stable Expression Cell Line (HuH-7) Copy Product Info
Catalog#： YOE-L062
Size： 1*10^6
Culture method： 90%DMEM+10% FBS
STR Report
Datasheet (Use Instruction)
  • 细胞图0 NAE1 Stable Expression Cell Line (HuH-7)-细胞图0
  • 细胞图1 NAE1 Stable Expression Cell Line (HuH-7)-细胞图1
  • 质粒图0 NAE1 Stable Expression Cell Line (HuH-7)-质粒图0
  • NAE1 Stable Expression Cell Line (HuH-7) NAE1 Stable Expression Cell Line (HuH-7)-产品图
NAE1 Stable Expression Cell Line (HuH-7) Copy Product Info
Catalog#： YOE-L062
Size： 1*10^6
Culture method： 90%DMEM+10% FBS
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Catalog YOE-L062 Transcript NM_001286500.2
Cell name NAE1 Stable Expression Cell Line (HuH-7) Species Human
Gene Name NAE1 Passage ratio 1:2~1:3
Cell Morphology Epithelial-like, adherent Antibiotic Concentration Puro=1μg/ml
Culture method 90%DMEM+10% FBS Mycoplasma test negative
Catalog
YOE-L062
Cell name
NAE1 Stable Expression Cell Line (HuH-7)
Gene Name
NAE1
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Transcript
NM_001286500.2
Species
Human
Antibiotic Concentration
Puro=1μg/ml
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS

Product Description

The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene.
Gene Expression Validation
No. Sample hNAE1-1CтMean β-h_actinCтMean ΔCтMean ΔΔCт Expression Rate
1 NAE1 Stable Expression Cell Line (HuH-7) 16.467 15.863 0.604 -3.898 14.908
2 Huh-7_Ctrl(Puro) 19.015 14.513 4.502 0.000 1.000
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:HuH-7		 STR Info (Cell bank)
Cell Line:HuH-7
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 10111011
D7S820 11111111
D16S539 10101010
VWA 16181618
TH01 7777
AMEL XXXX
TPOX 811811
CSF1PO 11111111
D12S391 2021
FGA 2223
D2S1338 1919
D21S11 3030
D18S51 1515
D8S1179 1414
D3S1358 1515
D6S1043 1315
PENTAE 1111
D19S433 1314
PENTAD 1212
D1S1656 1616
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    High expression
    Exclusive cell selection technique, ensuing high expression, RT-qPCR validation data provided.
  • icon
    Good stability
    Stable gene copy number, low passage, can be stably passaged for more than 20 passages.
  • icon
    Strict QC
    Strict quality control, ensuing no bacterial and mycoplasma contamination, STR authentication and cell post-thawing viability test passed; High standard delivery.
Citations:
Product Catalog#：YOE-L062
Product Name：NAE1 Stable Expression Cell Line (HuH-7)
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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